CD8

1. annotation.

1.1 Classical markers

T cells' annotation can be highly rely on the pubulication from zemin zhang's lab. the following reseach provide a detailed marker list for CD8 and CD4 T cells.
Lineage tracking reveals dynamic relationships of T cells in colorectal cancer | Nature

Extended Data Fig. 5: Summary of functional properties of various T cell clusters.

Pan-cancer single-cell landscape of tumor-infiltrating T cells | Science

Fig. S5. Signature genes of meta-clusters. (CD8)

Fig. S5. Signature genes of meta-clusters. (cd4)

This pubulication also provide a website for us to explorer their data: cancer-pku.cn:3838/PanC_T/

1.2 Other function related genes

METRNL: The cytokine Meteorin-like inhibits anti-tumor CD8+ T cell responses by disrupting mitochondrial function: Immunity.
DUSP4: An integrative analysis of the single-cell transcriptome identifies DUSP4 as an exhaustion-associated gene in tumor-infiltrating CD8+ T cells | Functional & Integrative Genomics. This genes seems highly expressed in hypoxia and stressed T cells. be careful.
SLAMF7: SLAMF7 Signaling Reprograms T Cells toward Exhaustion in the Tumor Microenvironment | The Journal of Immunology | American Association of Immunologists
IKZF1: Degradation of IKZF1 prevents epigenetic progression of T cell exhaustion in an antigen-specific assay: Cell Reports Medicine
T-bet (TBX21): High T-bet expression induced SLECs and low expression promoted MPECs.. SLEC (short-lived effector cells, IL7R^lo/TBX21^hi), MPECs (memory precursor effector cells, IL7R^hi). T exhaustion cells had a higher ratio of nuclear Eomes:T-bet than memory T cells (TMEMs) during chronic lymphocytic choriomeningitis virus (LCMV) infection in preclinical cancer models and in human tumors. which means T-bet supress T exhaustion, while EOMES promotes it.
Moreover, the short-live CD8 Teffect can be identified using markers below:

CD8+ T Cell Exhaustion in Cancer (fimmu.2021.715234)

2. Biology tips

2.1 Differentiation.

Bone marrow existed plenty of CD8 T effct cells, especially in paitens burndened with cancer Bone Marrow Contains Melanoma-reactive CD8+ Effector T Cells and, Compared with Peripheral Blood, Enriched Numbers of Melanoma-reactive CD8+ Memory T Cells. Actually, in homestasis, CD8Teff mainly become mature in thymus, not bone marrow.

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The origin of these CD8Teff mainly because of their homing to BM.
The bone marrow: a nest for migratory memory T cells: Trends in Immunology

short-live effect T cells and long live memory T cells